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The association of cigarette smoking with serum TSH concentration and thyroperoxidase antibody Exp Clin Endocrinol Diabetes

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RT-PCR RNA was extracted from fully matured HBE cells with the AllPrep DNA/RNA Mini kit (80204, QIAGEN AG, Germantown, MD) and treated with DNase I (M0303S, New England BioLabs Inc., Ipswich, MA) before reverse transcription with the iScript cDNA Synthesis Kit (1708891, Bio-Rad)