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The increased ILC3 frequencies in asthma patients correlate with asthma severity To understand the clinical significance of the increased ILC3 frequencies in smoking asthma patients, we analyzed the relationships between the frequencies of sputum ILC3s and blood CD4 + CD45RO + ILC3s and asthma severity, as measured by (i) the pulmonary function indices FEV 1 and FEV 1 /FVC ratio or (ii) asthma control, as measured by the ACQ and ACT scores

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The expression levels of each gene were normalized to the level of Rps3 expression for each sample