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One study, published in the journal Addictive Behaviors in 2022, shed new light on the reasons by showing that the first day of quitting is more challenging for women than for men

2.9 Differential expression analysis Differential expression analysis to determine marker genes for a cell cluster compared to all remaining cells was calculated via the FindAllMarkers function while pairwise comparisons were performed using the FindMarkers function

as the smoke seems to be tamed by the high density filter

Otherwise, check for anything else that doesn't work