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While tobacco twists are strange looking today, they were one of the most common forms of tobacco in the 1800s

We are continuing to require the date-stamp on both photos to ensure program integrity in the virtual setting

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For cleaved Caspase-3 IF detection, slides were incubated overnight with primary cleaved Caspase-3 antibody (Proteintech, 25128-1-AP, 1:200) at 4 C and later treated with DAPI and Cy3 secondary antibody (Abcam, ab97075, 1:200) for 1 h in the dark