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To assess the color gradient within a brand family as tar yields reduced, a between-pack E score was also calculated comparing each pack to the member of the brand family with the highest tar level (Marlboro Smooth and Peter Jackson Original, respectively)

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Duke also hired one of Bonsack's mechanics, resulting in fewer breakdowns of his machines than his competitors

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The RPE-choroid complex was dissected and permeabilized with 1% Triton for 12 h and then incubated in a blocking buffer (3% BSA, 0.3% Triton PBS) at room temperature for another 12 h