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The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h

Science 366 , 339345 (2019)

substitution of tobacco with alternative diluent materials

Your skin takes on a healthier glow thanks to better hydration and reduced inflammation

Estimates of gene flow were generated from an analysis that assessed evidence for recent and historical migration based on the analyses of 10,000 SNPs between these populations, which indicated negligible levels of connectivity consistent with long-term isolation and independent demographic histories