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Smoking weakens sperm, which can impact these measurements and reduce sperm quality

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23225) in each sample, the identical amounts of total proteins were loaded onto Mini-PROTEIN TGX TM Gels (BIO-RAD, Hercules, CA, catalog no.456-1083 or 456-1086) with 1X Tris/Glycine/SDS buffer (BIO-RAD, catalog no.1610732)