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CMMB were washed 3 times with 80% ethanol then resuspended with 50 l of 50 mM TEAB (Triethylammonium bicarbonate buffer)

Also, because concentrations of DA, AP and AC decline considerably in e-liquids over a 64 day period (Vas et al., 2019) the experimental design allowed for transfer efficiency to be examined across a range of compound concentrations that were consistent with previously reported ketone emissions from e-cigarettes (Farsalinos et al., 2015, Supplementary Table S1)

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Please note that you shouldnt select your quit day too much farther in the future since you may forget your goal and lose motivation along the way

Ingredients: 6MN, Black Tea Leaves, Salt, Sodium Carbonate, Saccharine, Propylene Glycol, Natural & Artificial Flavors, Xylitol, Vitamin E