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A person may not experience symptoms when they first develop cataracts but as the condition progresses, multiple symptoms that may occur such as blurred or cloudy vision, sensitivity to bright lights and double vision

The RRLuc-His sequence was then amplified using primers 20 and 21 and inserted into the NcoI and KpnI sites of pIVEX1.3_eYFP-His to generate pIVEX1.3_RRLuc-His