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Briefly, slides were treated with 0.4% pepsin (DAKO) at 37C for 30 min, incubated with primary antibody to alpha7 subunit of nAChR(Sigma, product number: N 8158) diluted 1:2000 in PBS containing 1% BSA at room temperature for 3 h, and incubated with simple mouse stain MAX-PO (RAT), a second antibody, at room temperature for 1 h

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