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The study subjects included men between 29 and 80 years old who underwent a comprehensive health checkup program, including esophagogastroduodenoscopy (EGD) and a workup for metabolic syndrome, at the clinic between January and June 2015

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Four-micrometer thick paraffin sections were deparaffinized in xylene and rehydrated in ethanol before hematoxylin and eosin staining for evaluation of whole tissue structure, Sirius red staining for eosinophils, periodic acid-Schiff and Alcian blue (PAS/AB) staining for goblet cells, and immunostaining