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Neutrophils were then washed in Hankss balanced salt solution and further purified using MACS-based neutrophil isolation kit (Milteyni Biotec130-097-658) as per manufacturers recommendation and cultured in serum-free AIM-V medium

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[1] Trusted information is essential for the safety of travellers who use nicotine products and will prevent them from unknowingly breaking the law or informal rules of behaviour