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For example, a 2021 review discussed possible ways NAC could help counter ROS-related survival, metastasis, and drug-resistance pathways in triple-negative breast cancer.39 And in a small uncontrolled pilot study involving women with breast cancer, NAC supplementation was associated with reduced markers of tumor cell proliferation.40 But animal research has found concerning effects in other contexts

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Therefore, it is necessary to follow strategies to minimize drug resistance and maximize antitumor activity (Wang et al., 2019)

Before termination of treatment, cells were incubated with 5 M MitoSOX for 15 min at 37 C, and washed in PBS