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Kinetic BRET measurements were taken at 37C using a Spark plate reader (Tecan) with 460/40 nm and 535/25 nm filters for RLuc8 and Venus emissions, respectively

For context, a parked car in direct sunlight can reach internal temperatures exceeding 122 degrees Fahrenheit in under an hour

If these procedures are not suitable or successful, assisted reproduction through sperm donation may also be a solution to fulfill the desire for children in cases of male infertility

These are mapped using the three dimensions of the UNESCO Historic Urban Landscape Approach: natural, built, and socio-cultural

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