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To justify testing, always include a medically necessary ICD-10 code such as: M81.0 (Osteoporosis) + E55.9 (Vitamin D Deficiency) N18.9 (Chronic Kidney Disease, Unspecified) + E55.9 Conclusion Accurate ICD-10 coding for Vitamin D deficiency is essential for proper diagnosis documentation, insurance reimbursement, and patient treatment plans

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However, there were notable differences in community composition between experiments including the absence of Akkermansia muciniphila , Bacteroides thetaiotaomicron , and Parasutterella species in the SURV experiments (Additional file 1 Fig