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Powell CM, Schoch S, Monteggia L, Barrot M, Matos MF, Feldmann N et al (2004)

Mammalian cell culture, transfection and generation of stable cell lines Mammalian cell cultures were performed following previously published protocols 6

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Besides their special organization in lipid domains (lipid rafts) of the membrane lipid bilayers, some sphingolipids, e.g., ceramides, sphingosine, and sphingosine-1-phosphate have been described to function as lipid-derived second messengers mainly regulating fundamental cellular pathways such as apoptosis and differentiation [119, 120]