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With the help of microplate reader, the changes in absorbance at 412 nm was measured within 5 min

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Biomarkers may be related to the disposition and metabolism of the drug (such as APAP protein adducts) or to the endogenous response of the host to the drug

Arthritis Rheumatol 67(11):28282836 Venkatesha SH, Astry B, Nanjundaiah SM, Kim HR, Rajaiah R, Yang Y, Tong L, Yu H, Berman BM, Moudgil KD (2016) Control of autoimmune arthritis by herbal extracts and their bioactive components