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The samples were vitrified after blotting for 3 sec using a Vitrobot Mark IV (FEI) with either 10 or 15 sec of incubation, blot force 0, at 100 % humidity, and 4 C

Without baseline data, it is impossible to determine whether a peptide is actually helping or whether natural disease fluctuation is responsible for any changes you observe

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In whole-cell current clamp experiments, hyperpolarizing or depolarizing current pulses (500 ms, from 10 to 30 pA in 5-pA steps) were injected into neurons to determine input resistances or trigger action potentials

Patients often begin from very different starting points