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Sperms were then released to an HTF drop on sperm capacitation dish (prepared by placing 200 L of HTF solution on a 35-mm dish to form a drop, followed by covering the drop with mineral oil and pre-balancing in a humidified incubator containing 5% CO 2 at 37 C for 12 h before use) by slightly pressing/squeezing the cauda epididymides, followed by incubation in a humidified incubator containing 5% CO 2 at 37 C for 0.5 h

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Astrocyte reactivity influences amyloid- effects on tau pathology in preclinical Alzheimers disease