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A 500mcg daily protocol under split dosing would look like: 300mcg upon waking (fasted), then 200mcg approximately 30 minutes before dinner or before bed on an empty stomach

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Lysosomal acid lipase-deficient mice: depletion of white and brown fat, severe hepatosplenomegaly, and shortened life span

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[13] Chloramphenicol has been shown to blunt reticulocyte response to cyanocobalamin, while ethanol, colchicine, and nitrous oxide interfere with cobalamin metabolism or utilization, each warranting clinician awareness when evaluating suboptimal treatment outcomes