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Real-Time Quantitative Reverse Transcription PCR (qRTPCR) Analysis Total RNA from skin tissues was isolated using TRIzol reagent (Sigma, Milwaukee, WI, USA), and 1 g of total RNA was reverse-transcribed into cDNA

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Approximately 10 5 cells of CO23, CO23MCFG and CO23RFLC strains were serially diluted and spotted onto YPD plates treated or not with 4 mM H 2 O 2 in absence (1) or in presence of GSH precursors 0.02 M glutamate, 0.02 M cysteine, 0.02 M, glycine (2) or with 5 mM BSO (L-buthionine-sulfoximine) (3)

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