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Crucially, Crat knockout experiments partially reversed these metabolic improvements, establishing CrAT-mediated SREBP1c inhibition as a key mechanism through which L-carnitine counteracts de novo lipogenesis

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Experimental Model and Specimen Collection A modified protocol named the win-PTZ kindling method has been established, in which animals received only four initial intraperitoneal (i.p.) injections of PTZ (37.5 mg/kg) every other day, followed by a time window where the animals didnt receive PTZ during the next 22 days, and thus the three last PTZ injections were done at the 29 th , 31 st , and 33 rd days

This may be partly due to lower total antioxidant activity in these patients, particularly the decrease in SOD and CAT activities (24, 25)

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