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A first strand complementary DNA (cDNA) synthesis kit (TAKARA, Dalian, China) was used for the synthesis of cDNA from RNA templates

Once the bottle of methyl crystals and the sterile saline is opened and mixed, the sterile integrity of the (now fluid) B12 can no longer be guaranteed and is therefore potentially open to contamination

While ketone bodies are physiologic molecules, their high levels reduce the physiological pH of the blood and induce ketoacidosis, leading to increasing metabolic dysfunction

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Half took semaglutide once weekly, the other took a placebo