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Animals 56 non-medicated, clinically healthy adult male Swiss albino rats (150 to 200 g) were gathered from the animal house of the Medical Research Center, Faculty of Medicine, Ain Shams University

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The obtained tissue powder was transferred to the Eppendorf probes and homogenised with 100 mM HCl, 2% (w/v) PVPP and 1 mM EDTA