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The studied dosage for AOD 9604 is 300 mcg/day

To induce barrier damage cells were incubated with proinflammatory cytokines (CK) tumor necrosis factor- (TNF-) and interleukin-1 (IL1-) both at 10 ng/ml concentration

Among the most important are (i) the TGF- signaling pathway that triggers downstream events such as the expression of connective tissue growth factor (CTGF), (ii) pathological mechanical forces (mechanotransduction) that occur through overload, repetitive stress or injury, (iii) pro-inflammatory cytokines and innate immunity in a persistent inflammatory environment, and (iv) excessive oxidative stress leading to cellular aging and senescence ( Figure 5 ) (90)

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