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The dermis was then carefully separated from the full-thickness skin specimens and soaked in collagenase solution (collagenase C7657 Sigma, 2 mgmL 1 ) at 37 C for 1 h with gentle agitation

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Other Measures To Consider If you are not eating oily fish at least twice a week, take supplemental fish oil, in capsule or liquid form (two to three grams a day of a product containing both EPA and DHA)

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Inhibition of GPR43/GPR109a expression by RNA interference Human G-protein-coupled receptor 43 (GPR43) or GPR109a siRNA or negative control (NC) siRNA was mixed with the Lipofectamine 2000 transfection reagent (Invitrogen, California, USA) (Table S1)