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Moreover, they obtained a decrease in average HbA1c of up to 2.2 percentage points

Waters) and separated using ultra HPLC on an Accela 3000 UHPLC (Thermo Fisher Scientific) using gradient elution at 0.5 ml min 1 and column temperature 55 C

Applying this approach, coefficients of variation of plasma reference samples that were run along with the NESDA samples met the original quality control criteria

ALT levels were determined spectrophotometrically at 450 nm and concentrations were calculated from an ALT standard curve (Tecan Reader infinite F201, Tecan)