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NTS prepared as previously described and cultured in M199 supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum were incubated at 37 C in a clear bottom flat well plate in a 5% CO 2 incubator overnight

Autonomic and Neurologic Symptoms The authors note that atypical clinical presentations of Lyme disease can occur, including gastrointestinal manifestations related to autonomic dysfunction , as was seen in this case

Rao JS, Harry GJ, Rapoport SI, Kim HW

Such non-monotonicity underscores the need for individualized titration and careful monitoring of psychiatric end points

An improvement in symptoms (urgency and frequency of urination) occurred as early as 2 weeks after the procedure