Straus, Roberta A
Roll the vial gently for several minutes
But I do uh, this idea, you know Id always thought about it, you know, totally independently that, you know, when-when Shinya Yamanaka, uh, discovered these y four transcription factors that could essentially take an old adult cell and revert it to an embryonic-like state, uh, it was like, wow
When the temperature was lowered to 20 C, the mixture was centrifuged at 10,000 g for 20 min, after which absorbance values were measured at 600, 450, and 532 nm
Filipowicz W, Bhattacharyya SN, Sonenberg N (2008) Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight