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Other studies have demonstrated that cancer cells that have undergone ferroptosis can release high mobility group Box 1 (HMGB1) in an autophagy-dependent manner (Yu et al., 2015

This concern is particularly relevant given the increasing popularity of off-label B12 use

Proper technique , site rotation , and sterility are essential to prevent injection site reactions and maintain safety

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Moreover, few studies have investigated off-target activation in normal tissues where GSH levels may still be substantial (e.g., liver, kidney)