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Each sample was then incubated with 2 L alkaline phosphatase (0.31 U/L) and 6 L Tris (100 mM, pH 7.5) for 1 h at 37 C

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The CUMS paradigm resulted in an elevation of pro-apoptotic proteins (Bax, caspase-3) and inflammatory markers (TNF-, IL-1), concurrently leading to a decrease in BDNF and GFAP levels