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Enzymes act as catalyststhey allow a reaction to proceed more rapidlyand they also allow the regulation of the rate of a metabolic reaction, for example, in response to changes in the cell's environment or to signals from other cells

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In addition, the activation of the transcription factor Nrf2 in HCECs upregulated AKR1C1 under hyperosmotic conditions

Total cellular proteins were extracted from cultured cells using a RIPA lysis buffer supplemented with phosphatase and protease inhibitors (Roche, Basel, Switzerland)