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After collection via centrifugation, the cells were incubated in PBS on ice for 10 min

After 12 treatment sessions, patients were assessed for pain intensity, knee flexion range of motion, a timed up-and-go test, a 6-min walk test, and knee functionality compared to pre-treatment baseline measurements

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In addition, bacterial extracellular vesicles (EV) mediate signal transmission between the gut microbiota and the host (Villard et al., 2021)

Unlike traditional treatments like PDE5 inhibitors (e.g., Viagra), which primarily target blood flow and can have side effects, PT-141 works by activating melanocortin receptors in the brain and central nervous system, specifically MC4R, to influence sexual desire and arousal