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Firstly, the use of BCPB results in clean chromatograms because the derivatization reaction does not yield byproducts and the detector analytical wavelength falls in the UV-region in which the biological matrix is usually transparent

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However, molecular biology techniques of targeted mutations using CRISPR/Cas, as well as functional studies of protein production and characterization, with activity assays, in addition to in silico and in vitro studies of protein-ligand or protein-protein interactions, could clarify the specific roles of these motifs (Ltoff et al., 1998