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Serum IGF-I levels alone are usually insufficient for diagnosing AGHD, so provocative tests such as the insulin tolerance test, glucagon stimulation test, or the combined GHRH-arginine test (where available) are needed (28)

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The plates were subsequently washed and incubated with biotin-labeled anti-IgG mAb (Mabtech, Sweden) and horseradish peroxidase-conjugated streptavidin (Mabtech, Nacka Strand, Sweden)