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Aggregation rather than shear is the specific risk with an amylin-family sequence: the prolines at positions 25, 28 and 29 reduce fibrillation propensity relative to native amylin but do not eliminate it, so inspect the solution against a light source for haze, opalescence or filaments before use

Efforts to detect these protein abnormalities in vivo have continued since the seminal work of George Glenner and Caine Wong in the 1980s, who successfully isolated and sequenced the A peptide from cerebral amyloid angiopathy [531]

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