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General cell culture and imaging The HeLa cell line used in this study was purchased from American Type Culture Collection (ATCC) and grown in DMEM (Gibco, 11965) media supplemented with 10% FBS and 1% 1003 Pen Strep (Gibco)

Patients with a history of pancreatitis or significant gastrointestinal issues should be particularly cautious, as incretin-based therapies like Tirzepatide may exacerbate these conditions

Importantly, tirzepatide's effect on gastric emptying may affect the absorption of oral medications, including oral contraceptives

History of pancreatitis requires careful risk-benefit assessment

This can be particularly beneficial for those suffering from chronic pain or inflammatory diseases