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Investigators use it to study the combined effects of BPC-157's cytoprotective pathways, TB-500's actin-regulated cell migration, KPV's melanocortin anti-inflammatory signaling, and GHK-Cu's copper-dependent collagen and antioxidant gene activation simultaneously
The titration schedule is different
The receptor was first incubated with the isolated G protein and, when applicable, the ligand (10 M) for 30 min in a 25 mM HEPES, 100 mM NaCl, 5 mM MgCl 2 , pH 7.5 buffer
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