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The investigation of the amino acid residues controlling the two activities (monophenolase and diphenolase) in PPOs and the quantification of their influence on the catalytic activity represent a tough challenge in this field and has remained unclear over the last decades

More important, the concentration of GLP-1(2836) required for cardioprotection (6 nM) could not possibly be generated by degradation of the 20-fold lower amounts of the parent GLP-1 used in our experiments (0.3 nM)

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