Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency
Growth was restored by supplementing the media with 1 mM GSH or with other thiols, such as mercaptoethanol, dithiothreitol or cysteine (Cuozzo & Kaiser, 1999)
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annuus leaves compared with the control ( Supplementary Figure S4 )
Upon HGF binding c-MET gets activated through kinase activity which is followed by receptor dimerization and trans-phosphorylation of two catalytic tyrosine residues Tyr1234 and Tyr1235 within the kinase activation region [31]