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Part of the challenge Jerne faced in determining the replication mechanism was he was an immunochemist and serologist so knew little about cells (Lefkovits, Waldmann, Aug 2025)

Untreated cells in 2% or 10% FBS DMEM for 3 days were used as negative controls

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The presence of small amounts of sugars (like sucrose) helps stabilize the peptide, as their hydrogen bonds mimic those of water and reduce moisture-related collapse