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The gastric cell culture medium was replaced with the appropriate co-culture medium, and the infected cells were incubated at 37C in a humidified 5% CO 2 incubator for 320 h as specified

The efficacy of ferroptosis inhibitors (e.g., GPX4 activators) or natural compounds in models of advanced DN (with established glomerular/tubulointerstitial damage) remains untestedwhether these interventions can reverse existing injury or only delay progression is unknown

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Consequently, longitudinal studies in humans are needed to determine the role of gut microbiota and TMAO in CVDs and facilitate the development of better therapeutic strategies