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siRNA knockdown experiments For siRNA transfection, 1.2 10 5 HeLa cells were seeded on culture plates, and transfected 24 h later with 40 pmol of control siRNA (1027281) or 20 pmol of each siRNAs against human zDHHC3 (SI02777642), zDHHC5 (SI04159694), zDHHC7 (SI00766850), and zDHHC11 (SI04365914) (all Qiagen) using TransIT-X2 transfection reagent (Mirus, MIR 6004)