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doi: 10.3389/fmicb.2025.1639438 Received 02 June 2025 Revised 17 November 2025 Accepted 17 November 2025 Published 12 February 2026 Volume 16 - 2025 Edited by Xue-Gong Li, Chinese Academy of Sciences (CAS), China Reviewed by Emmanuel Oliver Fenibo, Hensard University, Nigeria Judy Malas, University of Illinois Chicago, United States Alef Dos Santos, Ume University, Sweden Updates Copyright 2026 del Moral, Siggs, Macey, Fox-Powell, Pearson and Olsson-Francis

Cell migration assay BxPC-3 and HPDE6-C7 cells were seeded into 24-well plates at a density of (510) 10 5 cells/mL and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum for 1624 h to form confluent monolayers

While microbial colonization does not influence the quantity of B cells at different developmental stages in the bone marrow and spleen, it may facilitate B cell maturation in peripheral immune organs, augment the proportion of mature B cells, and enhance the diversity of the B cell receptor (BCR) repertoire

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