We further corroborated these effects in FSP1-deficient A375 and MDA-MB-231 cell lines
Transfection efficiency was detected by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blot 48 h after transfection and compared with cells transfected with the corresponding negative control vector or negative control siRNA
Annals of Translational Medicine, 5 (16), 324
Current studies have shown that 1) anomalous high SUA level is likely to induce a series of cardiovascular diseases, and acts as an independent risk factor for cardiovascular diseases, including atherosclerosis, hypertension, and coronary heart disease (Jingzhe Han et al., 2019)
A 2025 pilot study published in PubMed confirmed short-term tolerance in 2 participants at 20mg intravenously, though the sample size remains too limited for definitive safety conclusions