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Culturing was performed in RPMI 1640 medium enriched with 10% fetal bovine serum, maintained at 37C in a humidified incubator with an atmosphere of 5% CO 2 and 95% air

The results show a significant increase in ROS in PBMC of patients with newly diagnosed Graves disease, while the patients treated with methimazole showed ROS levels similar to those of euthyroid individuals

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In addition, other studies have also demonstrated the involvement of TRP channels in paclitaxel-induced pain, which are well known to be predominantly expressed on this subpopulation of DRG neurons