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Annema W, von Eckardstein A

After rinsing, the glass coverslip was transferred to the recording chamber mounted on an inverted microscope (Leica, DMIRB) and continuously superfused with the extracellular medium described above without adding MgSO4 (Mg 2+ ions free medium)

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Weeks 12 onward: Continued progress with the potential for 15% or more total body weight loss by 6 months for patients who stay on program

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