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Proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and blotted with anti-hCystatin C (MAB1196 [R&D, Minneapolis, MN] used at 500 ng/ml), and developed by enhanced chemiluminescence (ECL, Thermo Fisher Scientific)

doi:10.1016/j.jneuroim.2014.06.025

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