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An interim analysis was planned after two thirds of the total events had accrued with clear stopping rules and an alpha spending function to account for this interim look (in fact, the interim analysis did not take place until 741 events, out of the planned 854, took place)
Baker LD, et al
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Differential scanning calorimetry (DSC) showed a large melting signal at approximately 0 C in DMEM/FBS/ZIL, clearly suggesting that a major part of the solution turned to ice, as is usually observed in slow-freezing conditions
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