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Before each experiment, cells were detached by trypsinization, counted using a hemocytometer, seeded in 24 well plates at 10,000 cells per well and incubated for 24 hrs prior to the initiation of each experiment

Furthermore, in a subset of subjects (n = 12), local skeletal muscle metabolism was also assessed using microdialysis

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The cardiovascular impact via TMAO needs larger and longer human trials

The brain-derived neurotrophic factor (BDNF) and its receptor, tyrosine kinase receptor B (TrkB), are essential and common mechanisms for the antidepressant effects of both the parent molecules of ketamines [i.e., S, R()ketamine, S(+)ketamine, and R()ketamine] and their active metabolites [i.e., (2R,6R)-hydroxynorketamine and of S(+)norketamine] (Yang et al., 2019)